Sponceram® Carriers
Doped inorganic Sponceram® carrier materials (patent pending) are exceedingly suited for culturing anchorage-dependent cells. Moulded into macroporous discs with material-derived microporosity, this carrier design results in extremely large and absorbent surfaces. Cells anchor fast and distribute themselves uni-formly on Sponceram® discs mounted in Z® RP Bioreactors. Formation of ECM is stimulated and cells start growing in a three-dimensional fashion, giving rise to tissue-like structures.
Sponceram® 20-90 disc (65 x 3,5 mm, 14 m² surface)
Cells cultivated on Sponceram® carrier materials develop specific features: Morphology, organiza-tion, division and differentiation of cells grown on Sponceram® are similar to those found in natural tissues. Cells remain vital and productive for months. Excellent post processing patterns are ob-served during the production of glycoproteins.
Microscopic image of a Sponceram® matrix (25-times magnification) / CHO-cells on Sponceram® matrix, 48 hours after inoculation (Giemsa-staining), 60-times magnification
ECM-embedded cells are removed from Sponceram® discs in vital condition by fast rotation of the discs in special enzyme solutions. Even at full confluency this method provides easy access to large quantities of vital cells. Tested protocols for a number of human cells exist.
Type Porosity Use
Sponceram® 20-90 rough Long-term culture,
cells producing much ECM
Sponceram® 30-90 fine slowly proliferating
cells, little ECM
Sponceram® 30-90 HA fine primary cells, autologous cells
Sponceram® 20-145 rough highly adherent cells
Sponceram® 30-145 fine

highly adherent cells, producing little ECM
In the table Sponceram® discs with adjusted properties for different cell types are listed. For suitability tests of different Sponceram® materi-als in petri dishes small sample discs are available upon request.
Hiper AG